Biofilm formation, icaABCD genes and agr genotyping of Staphylococcus aureus from fish and ground beef

Abstract A total of 46 Staphylococcus aureus isolates from fish and ground beef were tested for the agr types, icaABCD genes, biofilm formation at 12, 25 37 °C by microtiter plate MTT assays. All positive icaABD while 97.8% icaC . produced biofilms °C, but 93.5% them also producers 12 °C. There was no significant difference in between using crystal violet assay ( P > 0.05). However, statistically differences detected as well < significantly different production all temperatures. Furthermore, a relationship presence genes observed. The type I most prevalent (54.4%) among isolates, followed II (41.3%) III (9.6%). In this study, S. exhibited ability responsible persistence bacteria foods, which may lead to food spoilage human health problems.